SYNTHESIS, CHARACTERIZATION, AND ANTIMICROBIAL ACTIVITY OF EXTRACTED CHITOSAN-BASED SILVER NANOPARTICLES

In recent years, nanoparticles of noble metals such as silver have gained enormous attraction owing to their distinct physical, chemical, and biological properties. the present study, (AgNPs) were synthesized from nitrate (AgNO3) using extracted chitosan reducing agent. The was exoskeleton marine crab Portunus pelagicus. Three different sample solutions prepared 0.02mM AgNO3 in 0.5% (w/v) chitosan, 0.04 mM 0.06 chitosan. Preliminary characterization formed made based on measurements ultraviolet-visible (UV-Vis) spectroscopy scanning electron microscopy (SEM). characteristic absorbance peak corresponding observed at 400nm. average size chitosan-based range 160-530nm. further tested for antimicrobial activity. exhibited effective antibacterial activity against Gram-negative bacteria Pseudomonas aeruginosa, Escherichia coli well Gram-positive Bacillus subtilis, Staphylococcus aureus, antifungal Candida albicans, Aspergillus flavus, Penicillium chrysogenum. Among concentrations solutions, a maximum zone inhibition all microbial cultures with produced 0.06mM solution. incorporated Cotton Gauze evaluate its It showed higher bactericidal aeruginosa followed by thus proved potential be used wound dressing material.